Genome skimming herbarium specimens for DNA barcoding and phylogenomics
نویسندگان
چکیده
منابع مشابه
Appraisal of the entire mitochondrial genome for DNA barcoding in birds
DNA barcoding based on a standardized region of 648 base pairs of mitochondrial DNAsequences from Cytochrome C Oxidase 1 (COX1) is proposed for animal species identification.Recent studies suggested that DNA barcoding has been effective for identifying 94% of birdspecies. The proposed threshold of 10 times the average intraspecific variation could be used forthe identification and delimitation ...
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Herbarium specimens are an important source of DNA for plant research but current sampling methods require the removal of material for DNA extraction. This is undesirable for irreplaceable specimens such as rare species or type material. Here I present the first non-destructive sampling method for extracting DNA from herbarium specimens. DNA was successfully retrieved from robust leaves and/or ...
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DNA extraction from plant tissues often causes most problems. For example, unsuccessful removal secondary metabolites during extraction, such as phenolic compounds in aromatic and medicinal plants, cause to some mistakes in result of molecular experiments by using of the extracted DNA. Achillea wilhelmsii is a medicinal plant belong to Asteraceae family and native to Iran, there is little infor...
متن کاملExtraction of ultrashort DNA molecules from herbarium specimens.
DNA extracted from herbarium specimens is highly fragmented; therefore, it is crucial to use extraction protocols that retrieve short DNA molecules. Improvements in extraction and DNA library preparation protocols for animal remains have allowed efficient retrieval of molecules shorter than 50 bp. Here, we applied these improvements to DNA extraction protocols for herbarium specimens and evalua...
متن کاملTechniques for Improving the Quality and Quantity of Dna Extracted from Herbarium Specimens
There is a need to modify DNA extraction methods to obtain amplifiable DNA from herbarium specimens that are relatively old –– we explored several methods to improve both quality and quantity of DNA obtained from a range of specimens of Asteraceae. Leaf tissue was sampled from herbarium specimens of varying ages. Modifications were made to the Qiagen DNeasy® Plant Mini Kit and the CTAB Extracti...
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ژورنال
عنوان ژورنال: Plant Methods
سال: 2018
ISSN: 1746-4811
DOI: 10.1186/s13007-018-0300-0